R2779
 |    |  |
 | Renin is processed as a 406 amino acid precursor (45,057 Da) containing a 23 amino acid signal peptide and a 43 amino acid propeptide. Native active renin is a 340 amino acid glycoprotein containing three disulfide bonds. |
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R2779
Sigma
Renin human
BioUltra, recombinant, expressed in HEK 293 cells, ≥90 unit/μg protein, ≥98% (SDS-PAGE)
| Enzyme Commission (EC) Number: | 3.4.23.15 ( BRENDA | IUBMB ) |
| MDL number: | MFCD00132171 |
Description
| Preparation Note | Expressed in HEK cells as prorenin. The prorenin is activated to renin using trypsin and re-purified. |
| Unit Definition | One unit will produce one fluorescent unit from the hydrolysis of Renin Substrate I (Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg) at 37 °C at pH 8.0 |
| Biochem/physiol Actions | Prorenin granules are stored within modified smooth muscle cells adjacent to the distal tubule and the afferent arteriole. Prorenin is released into the blood from the juxtaglomerular cells in the wall of the afferent arteriole. Prorenin is then hydrolyzed by plasma kallekrein to produce active renin. |
| Physical properties | Supplied as a solution in 100mM sodium acetate pH 4.2 |
| Renin is processed as a 406 amino acid precursor (45,057 Da) containing a 23 amino acid signal peptide and a 43 amino acid propeptide. Native active renin is a 340 amino acid glycoprotein containing three disulfide bonds. |
Properties
| recombinant | expressed in HEK 293 cells |
| grade | BioUltra |
| assay | ≥98% (SDS-PAGE) |
| Gene Information | human ... REN(5972) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
